Spectrophotometer is also called spectrometer, which is a scientific instrument that decomposes complex light into spectral lines. Using spectrophotometry, the spectrophotometer measures the absorbance of the measured substance at a specific wavelength or within a certain wavelength range to perform qualitative and quantitative analysis of the substance. The basic principles of different types of spectrophotometers are similar. They all use a light source that can generate multiple wavelengths, through a series of light splitting devices, to form a light source of a specific wavelength. After the light source passes through the test sample, part of the light source is absorbed. Subsequently, by measuring the absorbance of the sample, it can be converted into the concentration of the sample after calculation. The absorbance of the sample is proportional to the concentration of the sample.

